Our laboratory obtains and manufactures monoclonal antibodies produced by hybridoma cell cultures (hybridomas). This service includes immunization of mice with immunogenes, following by hybridoma generation, screening and subcloning, antibody production in hybridoma cell culture, purification and characterization. The idea of hybridoma technology consists of creating a hybrid cell, which is produced by way of fusing an antibody-producing B-cell with a myeloma cancer cell. Such hybridoma possesses the ability of a B-cell to secrete antibodies and the ability of a cancer cell to persistently reproduce itself. The splenocytes are used as a donor of antibody-producing B-cells, and the cells of the cultivated Sp2/0-Ag14 mouse myeloma cell line are used as cancer cells (use of another cell line is possible).