Patent of invention. Escherichia coli bacteria strain – producer of recombinant flagellin

17 March 2021

Al-Shekhadat Ruslan Ismailovich, Matjunina Ekaterina Aleksandrovna, Sharafutdinova Tatiana Ajratovna, Kozlov Andrej Petrovich, Klimov Nikolaj Anatolevich, Petrov Aleksandr Vladimirovich, Simbirtsev Andrej Semenovich.

Type: patent of invention
Patent number: RU 2524133 C2
Patent Office: Russia
Publication year: 2014
Application number: 2012148482/10
Date of registration: 15.11.2012
Date of publication: 07/27/2014


Substance: invention relates to biotechnology and a recombinant strain of Escherichia coli bacteria – a producer of biologically active flagellin. The described strain is obtained by transformation of an E. coli BL21[DE3] cell culture with recombinant plasmid DNA pET151FliC, which is obtained based on a pET151FliC vector in which was embedded a fliC gene which codes biologically active flagellin, having a nucleotide sequence represented in Seq ID No 3. The strain is deposited in the Russian National Collection of Industrial Microorganisms (RCIM) of the Research Institute for Genetics and Selection of Industrial Microorganisms under No B-11369.

Effect: the present solution has a higher production capacity with respect to recombinant flagellin, which is an effective adjuvant.

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