Creating protein overexpression strains
Custom recombinant protein production service

SARC-CoV-2 N protein

We can produce any recombinant proteins of high quality

Complete service: from gene synthesis (up to 8 kbp) to protein purification
All expression systems: bacterial, yeast, mammalian cells, insect cells
The purity of our proteins
more than 95 %
Bulk production
- from milligrams to grams
Any proteins: VHH and scFv antibodies, enzymes, antigens, receptors

Cost and production time

Contact us now to get quotes for your project!

The cost of production protein is negotiated on an individual basis.

The manufacturing process takes from 3 weeks.

How we work

1.Negotiations
with the customer

  • Determining
    the intended use
  • Reviewing the literature
  • Bringing in several options
  • Formulating the specification
  • Concluding the contract

2.Creating
a producer

  • Designing
    the amino acid sequence with the use of bioinformatical analysis, 3D modeling
  • Performing gene synthesis and creating the expression structure
  • Creating a strain (line) of the producer
  • Verifying the level of expression

3.Chromatographic
treatment

  • Implementing
    protein isolation using tag
  • Developing the technology for chromatographic purification of recombinant protein
  • Performing a refolding procedure if necessary

4.Quality
control

  • Assessing
    protein purity and identity using mass spectrometry and western blotting methods
  • Determining the concentration
  • Identifying the level of endotoxin
  • Determining the proteins and the DNA of the producer strain

5.Protein
activity measurement

  • Developing a method and determining the activity of the purified recombinant protein
  • Assessing protein activity
  • Compiling the report and the product datasheets
  • Sending the protein and producer to the Customer

Examples
of the recombinant proteins and producer strains

Modeling of the structure of flu virus hemagglutinin has been performed, a transmembrane domain has been deleted
Modeling of the structure of flu virus hemagglutinin has been performed, a transmembrane domain has been deleted
Gene optimization has been performed for better expression of recombinant protein in the cells of E. coli. 800 % increase in expression achieved
+IPTG
IPTG
+IPTG
time after induction
+IPTG
IPTG
time after induction
Gene optimization has been performed for better expression of recombinant protein in the cells of E. coli. 800 % increase in expression achieved - example 1
+IPTG
time after induction
Gene optimization has been performed for better expression of recombinant protein in the cells of E. coli. 800 % increase in expression achieved - пример 1
The overexpression strain has been created with the level of expression of recombinant protein of >50 %. The technology of recombinant protein isolation and purification has been developed.
The degree of purity amounted to >98 %
The overexpression strain has been created with the level of expression of recombinant protein of >50 %. The technology of recombinant protein isolation and purification has been developed.
The degree of purity amounted to >98 %  - example 1
The overexpression strain has been created with the level of expression of recombinant protein of >50 %. The technology of recombinant protein isolation and purification has been developed.
The degree of purity amounted to >98 %  - пример 1

The full range of services for recombinant proteins production

3D modeling of the structure of chimeric proteins

Creating expression constructions

Obtaining the strain (line) of recombinant protein producer

Optimizing the conditions for cultivation and induction of expression

Accumulating the biomass of the producer strain in the fermenters from 2 l up to 1,000 l

Developing and optimizing the technology of recombinant protein isolation and purification

Developing and optimizing the technology of recombinant protein refolding

Confirming the identity of recombinant protein using mass spectrometry and western blotting methods

Developing the method for determination of specific activity of recombinant protein (enzymatic, etc.)

Developing the methods for recombinant protein quality control

Scaling the technology for recombinant protein accumulation, isolation and purification

Developing the laboratory procedure for recombinant protein production

Developing the pilot industrial technology for recombinant protein production

Transfer of the technologies to the laboratory and production sites of the Customer

Supplying reagents and equipment, launch, adjustment and installation works for the developed technology line

Why should you work with us?

1.Strong international
academic and
industrial experience

2.Delivering
free of charge
consultation
on project

3.Guaranteed
protein xpression
and purification

4.Fast turnaround
time

5.Worldwide delivery
with DHL cold chain

Request a quote or discuss your project now!

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